by Jack Trout, Steve Rivkin. A newly revised and expanded edition of the revolutionary business classic, Differentiate or Die, Second Edition shows you how to differentiate your products, services, and business in order to dominate the competition. Veteran marketing guru Jack Trout. Start by marking “Differentiate or Die: Survival in Our Era of Killer Competition” as Want to Read: Giữa một rừng sản phẩm tương tự nhau, sự khác biệt của mỗi thương hiệu chính là phương cách duy nhất giúp bạn tạo ra dấu ấn trong tâm. After having read 'Positioning' by Al Ries and Trout, I was intrigued to read ' Differentiate or Die' by Jack Trout especially because of its title.
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Editorial Reviews. adirknesmala.gq Review. There are no two ways about it with Jack Trout. Die: Survival in Our Era of Killer Competition - site edition by Jack Trout. Download it once and read it on your site device, PC, phones or tablets. Differentiate or Die and millions of other books are available for site .. They successfully did what this book describes in beautiful and easy to read detail. Editorial Reviews. From the Inside Flap. Consumers today have an endless number of choices Download it once and read it on your site device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while.
The Creeping Commoditization of Categories. Whatever Happened to the Unique Selling Proposition?
Reinventing the Unique Selling Proposition. Creativity Is Not a Differentiating Idea. Price Is Rarely a Differentiating Idea. Preference Is a Differentiating Idea.
Hotness Is a Way to Differentiate. Growth Can Destroy Differentiation.
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Differentiation Often Requires Sacrifice. Being Different in Different Places. Maintaining Your Difference. Certain tumor cells upregulate HKII expression due to its higher affinity for glucose and its privileged location in the outer mitochondrial membrane [ 12 ].
Of course other key metabolic players should be considered, such as Hypoxia inducible factor-1alpha HIF-1a and c-Myc [ 7 , 15 — 17 ]. In fact, tumor aggressiveness and progression have been shown to positively correlate with a hypoxic microenvironment due to a high activity of HIF-1a and c-Myc [ 18 , 19 ] enhancing the transcription of genes coding for glycolytic enzymes and other important signaling pathways that help promote aerobic glycolysis, or the Warburg effect [ 15 , 17 , 20 ]. Taken together these data suggest that pharmacological strategies linked to the targeting of metabolic characteristics that define active cancer cells may also be useful in modulating pluripotent stem cell fate.
Although it may also have other targets, 3-brompyruvate 3BrP is a chemical pyruvate analog that functions as a potent inhibitor of glycolytic enzymes, most notably, but not exclusively, HKII [ 21 , 22 ].
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Importantly 3BrP has been used as an anti-cancer drug, including in clinical trials, and shown to induce cell death and thus reducing tumor size [ 2 , 23 , 24 ]. However, the exact mechanism by which this compound acts as an anti-tumor drug, is not completely known, although 3BrP treatment alters mitochondrial function in terms of both reactive oxygen species ROS and ATP production [ 25 ].
Given that ESCs are also highly proliferative, and share some common features with tumor cells, we wondered if 3BrP would also affect ESC pluripotency and could, in addition, be used as a practical and inexpensive tool to modulate stem cell fate by promoting differentiation by inducing a metabolic glycolysis-OXPHOS shift.
The goal of the present work was not to determine the importance of specific glycolysis enzymes towards the maintenance of pluripotency, for which a totally different experimental approach would be necessary, but rather to establish if the specific compound 3BrP could be a viable alternative for cell culture metabolic modulation in order to obtain specific cell lineages. E14Tg2a mESC were passaged when the right confluence was achieved, usually two or three days after platting.
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Briefly, 0. It is important to stress that all 3BrP experiments were carried out under strict pluripotency conditions i. The exception was the embryoid body experiments, specific for differentiation, as noted below. PI only enters cells with compromised membrane integrity, thus staining the nucleus of dead cells red.
Briefly, cells were collected at the 60h time point by enzymatic dissociation with accutase Gibco LIFe Technologies , and centrifuged for 5 min at rpm. Viability was assessed counting cells per condition; green fluorescent cells without red fluorescence were counted as live cells and a cell with both stains as dead cells using a fluorescent microscope.
Cells were plated and incubated for 60h and media was changed so that the assay did not take place in the presence of 3BrP. Intensity was measured colorimetrically at nm. Raw data was normalized to total cell number for each condition and then metabolic activity was normalized to the control. Alkaline phosphatase AP assay It is well accepted that stem cells that are self-renewing and pluripotent present high levels of AP [ 29 ]. To get the free app, enter your mobile phone number.
Consumers today have an endless number of choices among productsthat are virtually identical. Short of slashing your prices andwrecking your margins, differentiating is the only way to gainmarket share and win. Since its original publication, Differentiate or Die has becomethe bible for marketers who must find a way to separate theirproducts and brands from the competition.
In this fully updated edition of Differentiate or Die, JackTrout and Steve Rivkin bring the book in line with all the newestideas and recent changes in marketing. The only way to truly differentiate yourselfis by marketing the product's uniquely valuable qualities.
Full of practical case studies that show great differentiationin action, including new case studies from Russia and China,Differentiate or Die, Second Edition, shows you how to tap intocore differentiating ideas like heritage, market leadership, andbeing first to emotionally connect customers to your products.
Inaddition, this revision includes new research on brandcommoditization, new insight on using buzz to your advantage, a newchapter on differentiating absolutely anything, and a detailed lookat the perils of line extension in today's marketplace. These areonly a few of the valuable, proven differentiation strategiesincluded in the book, giving you unparalleled insight into one ofmarketing's most intractable problems.
From the classic "unique selling proposition" approach to theins and outs of product positioning, this book contains all thebest, most effective strategies for differentiating your productfrom the competition in tight quarters.
He isrecognized as a top marketing guru and is the creator of therevolutionary concept of positioning. He is also the coauthor ofthe bestselling classic Positioning, among many other titles. He iscoauthor of five books on marketing and communication strategy andis a frequent speaker at seminars and conferences around theworld.
Differentiation has become a very big word in business thanks ingreat part to Differentiate or Die. It has been called one of thebest marketing books of all time. Because of its importance, Jack Trout and Steve Rivkin haveupdated it and added new material they wish they had written thefirst time around.
Now it's better than ever, with eighteen new case studies,completely updated material, and three new chapters. One offersextensive research on category commoditization. Another coversdifferentiation in the new world of buzz.
The third new chapterreveals how you can differentiate anything using compelling,nonbusiness examples to prove this point. If you've read the First Edition, you'll probably enjoy this newSecond Edition even more.
For a full understanding ofdifferentiation today, keep this purple copy on your shelf next tothe original red one. Would you like to tell us about a lower price? A newly revised and expanded edition of the revolutionary business classic, Differentiate or Die, Second Edition shows you how to differentiate your products, services, and business in order to dominate the competition.
Veteran marketing guru Jack Trout uses real-world examples and his own unique insight to show you how to bind customers to your products for long-term success and loyalty.
This edition includes new case studies, new research, and updated examples from around the world. Read more Read less. Enabled Enhanced Typesetting: Enabled Page Flip: site Cloud Reader Read instantly in your browser. What other items do customers download after viewing this item? Al Ries. Differentiate or Die site Edition. Jack Trout. The 22 Immutable Laws of Marketing: Building a StoryBrand: Donald Miller.
Editorial Reviews From the Inside Flap Consumers today have an endless number of choices among productsthat are virtually identical. Announcing an update of one of the best marketing books of alltime.
See all Editorial Reviews. Product details File Size: Wiley; 2 edition December 28, Publication Date: December 28, Sold by: English ASIN: Enabled X-Ray: Not Enabled. Book Series.
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Share your thoughts with other customers. Write a customer review. Read reviews that mention jack trout differentiate or die immutable laws years ago must read differentiating idea laws of marketing required reading even though trout and steve rivkin many companies great book steve rivkin selling proposition position yourself in the marketplace everyone else nothing different unique selling long time good job.
Showing of 67 reviews. Top Reviews Most recent Top Reviews. There was a problem filtering reviews right now.The other thing that is hurting is that he is trying to give advice in areas where he has limited knowledge and experience. Enhanced Typesetting: If you've read the First Edition, you'll probably enjoy this newSecond Edition even more. Doug Stephens. Here is an excerpt: "Most publishers build their site by stapling together products made by other companies.
I had to struggle to finish this one. In this environment, distinctive product attributes are quickly copied by competitors, perceived by consumers to be minimal, or both.